Construct: shRNA TRCN0000235999

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018793.2-3856s21c1
DNA Barcode:
AGGCCTCCACACACCTTATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tyk2 (54721)
Gene Description:
tyrosine kinase 2
Transcript:
RefSeq NM_018793.2 (CURRENT)
Match location:
Position 3856 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235999)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGCCTCCACACACCTTATAC
Hairpin Sequence:
5'-CCGG-AGGCCTCCACACACCTTATAC-CTCGAG-GTATAAGGTGTGTGGAGGCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGCCTCCACACACCTTATACCTCGAGGTATAAGGTGTGTGGAGGCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGCCTCCACACACCTTATACCTCGAGGTATAAGGTGTGTGGAGGCCT-3'

Other clones with same target sequence:

(none)