Construct: shRNA TRCN0000236005

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021605.3-339s21c1
DNA Barcode:
TGGAGTGCCGGTAGCGTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nek7 (59125)
Gene Description:
NIMA (never in mitosis gene a)-related expressed kinase 7
Transcript:
RefSeq NM_021605.3 (NON-CURRENT)
Match location:
Position 339 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236005)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGAGTGCCGGTAGCGTTAAA
Hairpin Sequence:
5'-CCGG-TGGAGTGCCGGTAGCGTTAAA-CTCGAG-TTTAACGCTACCGGCACTCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGAGTGCCGGTAGCGTTAAACTCGAGTTTAACGCTACCGGCACTCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGAGTGCCGGTAGCGTTAAACTCGAGTTTAACGCTACCGGCACTCCA-3'

Other clones with same target sequence:

(none)