Construct: shRNA TRCN0000236028

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001003715.2-268s21c1
DNA Barcode:
TGCGACCATGGCTGTAGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RECQL5 (9400)
Gene Description:
RecQ like helicase 5
Transcript:
RefSeq NM_001003715.2 (NON-CURRENT)
Match location:
Position 268 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236028)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCGACCATGGCTGTAGTAAA
Hairpin Sequence:
5'-CCGG-TGCGACCATGGCTGTAGTAAA-CTCGAG-TTTACTACAGCCATGGTCGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCGACCATGGCTGTAGTAAACTCGAGTTTACTACAGCCATGGTCGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCGACCATGGCTGTAGTAAACTCGAGTTTACTACAGCCATGGTCGCA-3'

Other clones with same target sequence:

(none)