Construct: shRNA TRCN0000236062

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018198.2-601s21c1
DNA Barcode:
CTTTGCGCTCAGACGAGTAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DNAJC11 (55735)
Gene Description:
DnaJ heat shock protein family (Hsp40) member C11
Transcript:
RefSeq NM_018198.2 (NON-CURRENT)
Match location:
Position 601 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236062)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTGCGCTCAGACGAGTAAC
Hairpin Sequence:
5'-CCGG-CTTTGCGCTCAGACGAGTAAC-CTCGAG-GTTACTCGTCTGAGCGCAAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTGCGCTCAGACGAGTAACCTCGAGGTTACTCGTCTGAGCGCAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTGCGCTCAGACGAGTAACCTCGAGGTTACTCGTCTGAGCGCAAAG-3'

Other clones with same target sequence:

(none)