Construct: shRNA TRCN0000236079

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000512.4-1322s21c1
DNA Barcode:
CTTCAGACAGGGCATTGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GALNS (2588)
Gene Description:
galactosamine (N-acetyl)-6-sulfatase
Transcript:
RefSeq NM_000512.4 (NON-CURRENT)
Match location:
Position 1322 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236079)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCAGACAGGGCATTGATTT
Hairpin Sequence:
5'-CCGG-CTTCAGACAGGGCATTGATTT-CTCGAG-AAATCAATGCCCTGTCTGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCAGACAGGGCATTGATTTCTCGAGAAATCAATGCCCTGTCTGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCAGACAGGGCATTGATTTCTCGAGAAATCAATGCCCTGTCTGAAG-3'

Other clones with same target sequence:

(none)