Construct: shRNA TRCN0000236092

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006927.3-4001s21c1
DNA Barcode:
GGCGGATTACTTAATACTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ST3GAL2 (6483)
Gene Description:
ST3 beta-galactoside alpha-2,3-sialyltransferase 2
Transcript:
RefSeq NM_006927.3 (NON-CURRENT)
Match location:
Position 4001 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236092)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCGGATTACTTAATACTTAA
Hairpin Sequence:
5'-CCGG-GGCGGATTACTTAATACTTAA-CTCGAG-TTAAGTATTAAGTAATCCGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCGGATTACTTAATACTTAACTCGAGTTAAGTATTAAGTAATCCGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCGGATTACTTAATACTTAACTCGAGTTAAGTATTAAGTAATCCGCC-3'

Other clones with same target sequence:

(none)