Construct: shRNA TRCN0000236105

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145177.2-1692s21c1
DNA Barcode:
CCTAACCCTCAGAACCTATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DHRSX (207063)
Gene Description:
dehydrogenase/reductase X-linked
Transcript:
RefSeq NM_145177.2 (NON-CURRENT)
Match location:
Position 1692 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236105)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAACCCTCAGAACCTATAA
Hairpin Sequence:
5'-CCGG-CCTAACCCTCAGAACCTATAA-CTCGAG-TTATAGGTTCTGAGGGTTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAACCCTCAGAACCTATAACTCGAGTTATAGGTTCTGAGGGTTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAACCCTCAGAACCTATAACTCGAGTTATAGGTTCTGAGGGTTAGG-3'

Other clones with same target sequence:

(none)