Construct: shRNA TRCN0000236127

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152411.3-2449s21c1
DNA Barcode:
GCCAACTGTTTGCAATGATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF786 (136051)
Gene Description:
zinc finger protein 786
Transcript:
RefSeq NM_152411.3 (NON-CURRENT)
Match location:
Position 2449 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236127)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAACTGTTTGCAATGATAG
Hairpin Sequence:
5'-CCGG-GCCAACTGTTTGCAATGATAG-CTCGAG-CTATCATTGCAAACAGTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAACTGTTTGCAATGATAGCTCGAGCTATCATTGCAAACAGTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAACTGTTTGCAATGATAGCTCGAGCTATCATTGCAAACAGTTGGC-3'

Other clones with same target sequence:

  1. TRCN0000433935