Construct: shRNA TRCN0000236224

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181441.2-536s21c1
DNA Barcode:
AGTACGAGCAGGAGCTCAGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MRPL55 (128308)
Gene Description:
mitochondrial ribosomal protein L55
Transcript:
RefSeq NM_181441.2 (CURRENT)
Match location:
Position 536 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236224)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTACGAGCAGGAGCTCAGTG
Hairpin Sequence:
5'-CCGG-AGTACGAGCAGGAGCTCAGTG-CTCGAG-CACTGAGCTCCTGCTCGTACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTACGAGCAGGAGCTCAGTGCTCGAGCACTGAGCTCCTGCTCGTACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTACGAGCAGGAGCTCAGTGCTCGAGCACTGAGCTCCTGCTCGTACT-3'

Other clones with same target sequence:

(none)