Construct: shRNA TRCN0000236231

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003639.3-1447s21c1
DNA Barcode:
TGTCCCAAGTGCCAGTATCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IKBKG (8517)
Gene Description:
inhibitor of nuclear factor kappa B kinase regulatory subunit gamma
Transcript:
RefSeq NM_003639.3 (NON-CURRENT)
Match location:
Position 1447 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236231)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTCCCAAGTGCCAGTATCAG
Hairpin Sequence:
5'-CCGG-TGTCCCAAGTGCCAGTATCAG-CTCGAG-CTGATACTGGCACTTGGGACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTCCCAAGTGCCAGTATCAGCTCGAGCTGATACTGGCACTTGGGACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTCCCAAGTGCCAGTATCAGCTCGAGCTGATACTGGCACTTGGGACA-3'

Other clones with same target sequence:

(none)