Construct: shRNA TRCN0000236234

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014977.3-4468s21c1
DNA Barcode:
ATCCCTACATACATACCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACIN1 (22985)
Gene Description:
apoptotic chromatin condensation inducer 1
Transcript:
RefSeq NM_014977.3 (CURRENT)
Match location:
Position 4468 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236234)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCCCTACATACATACCAAAT
Hairpin Sequence:
5'-CCGG-ATCCCTACATACATACCAAAT-CTCGAG-ATTTGGTATGTATGTAGGGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCCCTACATACATACCAAATCTCGAGATTTGGTATGTATGTAGGGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCCCTACATACATACCAAATCTCGAGATTTGGTATGTATGTAGGGAT-3'

Other clones with same target sequence:

(none)