Construct: shRNA TRCN0000236244

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024733.3-1253s21c1
DNA Barcode:
ATGCATTCAAACTTAACTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF665 (79788)
Gene Description:
zinc finger protein 665
Transcript:
RefSeq NM_024733.3 (NON-CURRENT)
Match location:
Position 1253 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236244)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGCATTCAAACTTAACTAAG
Hairpin Sequence:
5'-CCGG-ATGCATTCAAACTTAACTAAG-CTCGAG-CTTAGTTAAGTTTGAATGCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGCATTCAAACTTAACTAAGCTCGAGCTTAGTTAAGTTTGAATGCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGCATTCAAACTTAACTAAGCTCGAGCTTAGTTAAGTTTGAATGCAT-3'

Other clones with same target sequence:

(none)