Construct: shRNA TRCN0000236257

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014283.3-4286s21c1
DNA Barcode:
TAAGGACAATGGTAGACATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SUCO (51430)
Gene Description:
SUN domain containing ossification factor
Transcript:
RefSeq NM_014283.3 (NON-CURRENT)
Match location:
Position 4286 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236257)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAAGGACAATGGTAGACATTT
Hairpin Sequence:
5'-CCGG-TAAGGACAATGGTAGACATTT-CTCGAG-AAATGTCTACCATTGTCCTTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAAGGACAATGGTAGACATTTCTCGAGAAATGTCTACCATTGTCCTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAAGGACAATGGTAGACATTTCTCGAGAAATGTCTACCATTGTCCTTA-3'

Other clones with same target sequence:

(none)