Construct: shRNA TRCN0000236357

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175709.3-155s21c1
DNA Barcode:
GAAGGGTAAAGTCGAGTATCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CBX7 (23492)
Gene Description:
chromobox 7
Transcript:
RefSeq NM_175709.3 (NON-CURRENT)
Match location:
Position 155 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236357)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGGTAAAGTCGAGTATCT
Hairpin Sequence:
5'-CCGG-GAAGGGTAAAGTCGAGTATCT-CTCGAG-AGATACTCGACTTTACCCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGGTAAAGTCGAGTATCTCTCGAGAGATACTCGACTTTACCCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGGTAAAGTCGAGTATCTCTCGAGAGATACTCGACTTTACCCTTC-3'

Other clones with same target sequence:

(none)