Construct: shRNA TRCN0000236361

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000966.4-963s21c1
DNA Barcode:
GTGCGAAATGACCGGAACAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RARG (5916)
Gene Description:
retinoic acid receptor gamma
Transcript:
RefSeq NM_000966.4 (NON-CURRENT)
Match location:
Position 963 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236361)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCGAAATGACCGGAACAAG
Hairpin Sequence:
5'-CCGG-GTGCGAAATGACCGGAACAAG-CTCGAG-CTTGTTCCGGTCATTTCGCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCGAAATGACCGGAACAAGCTCGAGCTTGTTCCGGTCATTTCGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCGAAATGACCGGAACAAGCTCGAGCTTGTTCCGGTCATTTCGCAC-3'

Other clones with same target sequence:

(none)