Construct: shRNA TRCN0000236391

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018107.4-2299s21c1
DNA Barcode:
GCCTCAACCCTGGCAATTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RBM23 (55147)
Gene Description:
RNA binding motif protein 23
Transcript:
RefSeq NM_018107.4 (NON-CURRENT)
Match location:
Position 2299 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236391)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCAACCCTGGCAATTATA
Hairpin Sequence:
5'-CCGG-GCCTCAACCCTGGCAATTATA-CTCGAG-TATAATTGCCAGGGTTGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCAACCCTGGCAATTATACTCGAGTATAATTGCCAGGGTTGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCAACCCTGGCAATTATACTCGAGTATAATTGCCAGGGTTGAGGC-3'

Other clones with same target sequence:

(none)