Construct: shRNA TRCN0000236435

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000720.2-7550s21c1
DNA Barcode:
ATATCCATGGGTCCTAATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CACNA1D (776)
Gene Description:
calcium voltage-gated channel subunit alpha1 D
Transcript:
RefSeq NM_000720.2 (NON-CURRENT)
Match location:
Position 7550 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236435)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATATCCATGGGTCCTAATAAT
Hairpin Sequence:
5'-CCGG-ATATCCATGGGTCCTAATAAT-CTCGAG-ATTATTAGGACCCATGGATAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATATCCATGGGTCCTAATAATCTCGAGATTATTAGGACCCATGGATATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATATCCATGGGTCCTAATAATCTCGAGATTATTAGGACCCATGGATAT-3'

Other clones with same target sequence:

(none)