Construct: shRNA TRCN0000236443

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018717.4-6259s21c1
DNA Barcode:
AGTACTGGGACCAACTAATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAML3 (55534)
Gene Description:
mastermind like transcriptional coactivator 3
Transcript:
RefSeq NM_018717.4 (NON-CURRENT)
Match location:
Position 6259 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236443)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTACTGGGACCAACTAATAG
Hairpin Sequence:
5'-CCGG-AGTACTGGGACCAACTAATAG-CTCGAG-CTATTAGTTGGTCCCAGTACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTACTGGGACCAACTAATAGCTCGAGCTATTAGTTGGTCCCAGTACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTACTGGGACCAACTAATAGCTCGAGCTATTAGTTGGTCCCAGTACT-3'

Other clones with same target sequence:

(none)