Construct: shRNA TRCN0000236461

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014681.4-1290s21c1
DNA Barcode:
TCGCTCTTCTCCAGCTATTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DHX34 (9704)
Gene Description:
DExH-box helicase 34
Transcript:
RefSeq NM_014681.4 (NON-CURRENT)
Match location:
Position 1290 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236461)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGCTCTTCTCCAGCTATTTC
Hairpin Sequence:
5'-CCGG-TCGCTCTTCTCCAGCTATTTC-CTCGAG-GAAATAGCTGGAGAAGAGCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGCTCTTCTCCAGCTATTTCCTCGAGGAAATAGCTGGAGAAGAGCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGCTCTTCTCCAGCTATTTCCTCGAGGAAATAGCTGGAGAAGAGCGA-3'

Other clones with same target sequence:

(none)