Construct: shRNA TRCN0000236467

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001271.3-4210s21c1
DNA Barcode:
TATCCGGAGTTCAGGTTAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHD2 (1106)
Gene Description:
chromodomain helicase DNA binding protein 2
Transcript:
RefSeq NM_001271.3 (NON-CURRENT)
Match location:
Position 4210 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236467)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATCCGGAGTTCAGGTTAATG
Hairpin Sequence:
5'-CCGG-TATCCGGAGTTCAGGTTAATG-CTCGAG-CATTAACCTGAACTCCGGATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATCCGGAGTTCAGGTTAATGCTCGAGCATTAACCTGAACTCCGGATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATCCGGAGTTCAGGTTAATGCTCGAGCATTAACCTGAACTCCGGATA-3'

Other clones with same target sequence:

(none)