Construct: shRNA TRCN0000236539

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015074.3-4060s21c1
DNA Barcode:
GTAGGTCGTATTCGGAATAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIF1B (23095)
Gene Description:
kinesin family member 1B
Transcript:
RefSeq NM_015074.3 (CURRENT)
Match location:
Position 4060 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236539)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGGTCGTATTCGGAATAAG
Hairpin Sequence:
5'-CCGG-GTAGGTCGTATTCGGAATAAG-CTCGAG-CTTATTCCGAATACGACCTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGGTCGTATTCGGAATAAGCTCGAGCTTATTCCGAATACGACCTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGGTCGTATTCGGAATAAGCTCGAGCTTATTCCGAATACGACCTAC-3'

Other clones with same target sequence:

(none)