Construct: shRNA TRCN0000236597

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001716119.1-1668s21c1
DNA Barcode:
ACAGGCCATGGTCCAGTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RBM20 (282996)
Gene Description:
RNA binding motif protein 20
Transcript:
RefSeq XM_001716119.1 (NON-CURRENT)
Match location:
Position 1668 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236597)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGGCCATGGTCCAGTATTA
Hairpin Sequence:
5'-CCGG-ACAGGCCATGGTCCAGTATTA-CTCGAG-TAATACTGGACCATGGCCTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGGCCATGGTCCAGTATTACTCGAGTAATACTGGACCATGGCCTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGGCCATGGTCCAGTATTACTCGAGTAATACTGGACCATGGCCTGT-3'

Other clones with same target sequence:

(none)