Construct: shRNA TRCN0000236614

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001722459.1-392s21c1
DNA Barcode:
AGAGTGCCCTTCTGATGAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC388720 (388720)
Gene Description:
similar to ubiquitin
Transcript:
RefSeq XM_001722459.1 (NON-CURRENT)
Match location:
Position 392 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236614)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGTGCCCTTCTGATGAATG
Hairpin Sequence:
5'-CCGG-AGAGTGCCCTTCTGATGAATG-CTCGAG-CATTCATCAGAAGGGCACTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGTGCCCTTCTGATGAATGCTCGAGCATTCATCAGAAGGGCACTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGTGCCCTTCTGATGAATGCTCGAGCATTCATCAGAAGGGCACTCT-3'

Other clones with same target sequence:

(none)