Construct: shRNA TRCN0000236678

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080424.1-921s21c1
DNA Barcode:
TTGAGCACAAACGGAACTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KDM6B (23135)
Gene Description:
lysine demethylase 6B
Transcript:
RefSeq NM_001080424.1 (NON-CURRENT)
Match location:
Position 921 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236678)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGAGCACAAACGGAACTATG
Hairpin Sequence:
5'-CCGG-TTGAGCACAAACGGAACTATG-CTCGAG-CATAGTTCCGTTTGTGCTCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGAGCACAAACGGAACTATGCTCGAGCATAGTTCCGTTTGTGCTCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGAGCACAAACGGAACTATGCTCGAGCATAGTTCCGTTTGTGCTCAA-3'

Other clones with same target sequence:

(none)