Construct: shRNA TRCN0000236693

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001716154.1-503s21c1
DNA Barcode:
AGGTTCAAGAACAGGTTAAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZSWIM6 (57688)
Gene Description:
zinc finger SWIM-type containing 6
Transcript:
RefSeq XM_001716154.1 (NON-CURRENT)
Match location:
Position 503 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236693)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGTTCAAGAACAGGTTAAAC
Hairpin Sequence:
5'-CCGG-AGGTTCAAGAACAGGTTAAAC-CTCGAG-GTTTAACCTGTTCTTGAACCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGTTCAAGAACAGGTTAAACCTCGAGGTTTAACCTGTTCTTGAACCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGTTCAAGAACAGGTTAAACCTCGAGGTTTAACCTGTTCTTGAACCT-3'

Other clones with same target sequence:

(none)