Construct: shRNA TRCN0000236699

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014758.2-866s21c1
DNA Barcode:
CCAGATGATTATTCGAGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNX19 (399979)
Gene Description:
sorting nexin 19
Transcript:
RefSeq NM_014758.2 (CURRENT)
Match location:
Position 866 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236699)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATGATTATTCGAGATTT
Hairpin Sequence:
5'-CCGG-CCAGATGATTATTCGAGATTT-CTCGAG-AAATCTCGAATAATCATCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATGATTATTCGAGATTTCTCGAGAAATCTCGAATAATCATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATGATTATTCGAGATTTCTCGAGAAATCTCGAATAATCATCTGG-3'

Other clones with same target sequence:

(none)