Construct: shRNA TRCN0000236762

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016801.3-484s21c1
DNA Barcode:
ACCACGACCAGTGAGGAATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stx1a (20907)
Gene Description:
syntaxin 1A (brain)
Transcript:
RefSeq NM_016801.3 (NON-CURRENT)
Match location:
Position 484 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236762)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCACGACCAGTGAGGAATTG
Hairpin Sequence:
5'-CCGG-ACCACGACCAGTGAGGAATTG-CTCGAG-CAATTCCTCACTGGTCGTGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCACGACCAGTGAGGAATTGCTCGAGCAATTCCTCACTGGTCGTGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCACGACCAGTGAGGAATTGCTCGAGCAATTCCTCACTGGTCGTGGT-3'

Other clones with same target sequence:

(none)