Construct: shRNA TRCN0000236772

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001480244.1-2069s21c1
DNA Barcode:
CATTACGAGCACACCTTATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zbtb11 (271377)
Gene Description:
zinc finger and BTB domain containing 11
Transcript:
RefSeq XM_001480244.1 (NON-CURRENT)
Match location:
Position 2069 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000236772)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTACGAGCACACCTTATTC
Hairpin Sequence:
5'-CCGG-CATTACGAGCACACCTTATTC-CTCGAG-GAATAAGGTGTGCTCGTAATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTACGAGCACACCTTATTCCTCGAGGAATAAGGTGTGCTCGTAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTACGAGCACACCTTATTCCTCGAGGAATAAGGTGTGCTCGTAATG-3'

Other clones with same target sequence:

(none)