Construct: shRNA TRCN0000237815

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001109662.1-398s21c1
DNA Barcode:
TGCGGAAGACACCCATATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HECTD4 (283450)
Gene Description:
HECT domain E3 ubiquitin protein ligase 4
Transcript:
RefSeq NM_001109662.1 (NON-CURRENT)
Match location:
Position 398 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000237815)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCGGAAGACACCCATATATA
Hairpin Sequence:
5'-CCGG-TGCGGAAGACACCCATATATA-CTCGAG-TATATATGGGTGTCTTCCGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCGGAAGACACCCATATATACTCGAGTATATATGGGTGTCTTCCGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCGGAAGACACCCATATATACTCGAGTATATATGGGTGTCTTCCGCA-3'

Other clones with same target sequence:

(none)