Construct: shRNA TRCN0000237835

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014159.5-1230s21c1
DNA Barcode:
TTCCGACGAGGGTCATCATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SETD2 (29072)
Gene Description:
SET domain containing 2, histone lysine methyltransferase
Transcript:
RefSeq NM_014159.5 (NON-CURRENT)
Match location:
Position 1230 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000237835)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCCGACGAGGGTCATCATAT
Hairpin Sequence:
5'-CCGG-TTCCGACGAGGGTCATCATAT-CTCGAG-ATATGATGACCCTCGTCGGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCCGACGAGGGTCATCATATCTCGAGATATGATGACCCTCGTCGGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCCGACGAGGGTCATCATATCTCGAGATATGATGACCCTCGTCGGAA-3'

Other clones with same target sequence:

(none)