Construct: shRNA TRCN0000237863

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020892.2-1728s21c1
DNA Barcode:
CAGAGCCAGAGCAGGTCATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DTX2 (113878)
Gene Description:
deltex E3 ubiquitin ligase 2
Transcript:
RefSeq NM_020892.2 (CURRENT)
Match location:
Position 1728 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000237863)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAGCCAGAGCAGGTCATAA
Hairpin Sequence:
5'-CCGG-CAGAGCCAGAGCAGGTCATAA-CTCGAG-TTATGACCTGCTCTGGCTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAGCCAGAGCAGGTCATAACTCGAGTTATGACCTGCTCTGGCTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAGCCAGAGCAGGTCATAACTCGAGTTATGACCTGCTCTGGCTCTG-3'

Other clones with same target sequence:

(none)