Construct: shRNA TRCN0000237882

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144997.5-2587s21c1
DNA Barcode:
TCAGTATGCAGTCGCAATAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FLCN (201163)
Gene Description:
folliculin
Transcript:
RefSeq NM_144997.5 (NON-CURRENT)
Match location:
Position 2587 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000237882)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAGTATGCAGTCGCAATAAC
Hairpin Sequence:
5'-CCGG-TCAGTATGCAGTCGCAATAAC-CTCGAG-GTTATTGCGACTGCATACTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAGTATGCAGTCGCAATAACCTCGAGGTTATTGCGACTGCATACTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAGTATGCAGTCGCAATAACCTCGAGGTTATTGCGACTGCATACTGA-3'

Other clones with same target sequence:

(none)