Construct: shRNA TRCN0000237885

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144997.5-1880s21c1
DNA Barcode:
CTCTCAGCAAGTACGAGTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FLCN (201163)
Gene Description:
folliculin
Transcript:
RefSeq NM_144997.5 (NON-CURRENT)
Match location:
Position 1880 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000237885)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTCAGCAAGTACGAGTTTG
Hairpin Sequence:
5'-CCGG-CTCTCAGCAAGTACGAGTTTG-CTCGAG-CAAACTCGTACTTGCTGAGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTCAGCAAGTACGAGTTTGCTCGAGCAAACTCGTACTTGCTGAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTCAGCAAGTACGAGTTTGCTCGAGCAAACTCGTACTTGCTGAGAG-3'

Other clones with same target sequence:

(none)