Construct: shRNA TRCN0000237908

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001717325.1-5524s21c1
DNA Barcode:
ACTGTCAAACTTACGTATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM184B (27146)
Gene Description:
family with sequence similarity 184 member B
Transcript:
RefSeq XM_001717325.1 (NON-CURRENT)
Match location:
Position 5524 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000237908)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGTCAAACTTACGTATTAA
Hairpin Sequence:
5'-CCGG-ACTGTCAAACTTACGTATTAA-CTCGAG-TTAATACGTAAGTTTGACAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGTCAAACTTACGTATTAACTCGAGTTAATACGTAAGTTTGACAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGTCAAACTTACGTATTAACTCGAGTTAATACGTAAGTTTGACAGT-3'

Other clones with same target sequence:

(none)