Construct: shRNA TRCN0000237942

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080541.1-11390s21c1
DNA Barcode:
TATAGCAGTTTCCCGTTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MGA (23269)
Gene Description:
MAX dimerization protein MGA
Transcript:
RefSeq NM_001080541.1 (NON-CURRENT)
Match location:
Position 11390 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000237942)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATAGCAGTTTCCCGTTATTT
Hairpin Sequence:
5'-CCGG-TATAGCAGTTTCCCGTTATTT-CTCGAG-AAATAACGGGAAACTGCTATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATAGCAGTTTCCCGTTATTTCTCGAGAAATAACGGGAAACTGCTATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATAGCAGTTTCCCGTTATTTCTCGAGAAATAACGGGAAACTGCTATA-3'

Other clones with same target sequence:

(none)