Construct: shRNA TRCN0000237960

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080454.1-1506s21c1
DNA Barcode:
TGTCAGTAGTCCAGATCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACSM4 (341392)
Gene Description:
acyl-CoA synthetase medium chain family member 4
Transcript:
RefSeq NM_001080454.1 (CURRENT)
Match location:
Position 1506 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000237960)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTCAGTAGTCCAGATCAAAT
Hairpin Sequence:
5'-CCGG-TGTCAGTAGTCCAGATCAAAT-CTCGAG-ATTTGATCTGGACTACTGACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTCAGTAGTCCAGATCAAATCTCGAGATTTGATCTGGACTACTGACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTCAGTAGTCCAGATCAAATCTCGAGATTTGATCTGGACTACTGACA-3'

Other clones with same target sequence:

(none)