Construct: shRNA TRCN0000237962

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001715003.1-5925s21c1
DNA Barcode:
GGAGATTACCTATGACTATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SETD1B (23067)
Gene Description:
SET domain containing 1B, histone lysine methyltransferase
Transcript:
RefSeq XM_001715003.1 (NON-CURRENT)
Match location:
Position 5925 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000237962)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAGATTACCTATGACTATAA
Hairpin Sequence:
5'-CCGG-GGAGATTACCTATGACTATAA-CTCGAG-TTATAGTCATAGGTAATCTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAGATTACCTATGACTATAACTCGAGTTATAGTCATAGGTAATCTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAGATTACCTATGACTATAACTCGAGTTATAGTCATAGGTAATCTCC-3'

Other clones with same target sequence:

(none)