Construct: shRNA TRCN0000237981

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025015.2-1132s21c1
DNA Barcode:
GGCGGACCCTATGGATCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HSPA12A (259217)
Gene Description:
heat shock protein family A (Hsp70) member 12A
Transcript:
RefSeq NM_025015.2 (NON-CURRENT)
Match location:
Position 1132 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000237981)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCGGACCCTATGGATCTTTA
Hairpin Sequence:
5'-CCGG-GGCGGACCCTATGGATCTTTA-CTCGAG-TAAAGATCCATAGGGTCCGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCGGACCCTATGGATCTTTACTCGAGTAAAGATCCATAGGGTCCGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCGGACCCTATGGATCTTTACTCGAGTAAAGATCCATAGGGTCCGCC-3'

Other clones with same target sequence:

(none)