Construct: shRNA TRCN0000237991

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081288.1-4338s21c1
DNA Barcode:
GTAGTACATTTGGTGATTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Taf2 (319944)
Gene Description:
TATA-box binding protein associated factor 2
Transcript:
RefSeq NM_001081288.1 (CURRENT)
Match location:
Position 4338 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000237991)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGTACATTTGGTGATTATG
Hairpin Sequence:
5'-CCGG-GTAGTACATTTGGTGATTATG-CTCGAG-CATAATCACCAAATGTACTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGTACATTTGGTGATTATGCTCGAGCATAATCACCAAATGTACTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGTACATTTGGTGATTATGCTCGAGCATAATCACCAAATGTACTAC-3'

Other clones with same target sequence:

(none)