Construct: shRNA TRCN0000238082

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181071.3-602s21c1
DNA Barcode:
ACAACCAAGTGACCAATATAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tanc2 (77097)
Gene Description:
tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 2
Transcript:
RefSeq NM_181071.3 (CURRENT)
Match location:
Position 602 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238082)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAACCAAGTGACCAATATAG
Hairpin Sequence:
5'-CCGG-ACAACCAAGTGACCAATATAG-CTCGAG-CTATATTGGTCACTTGGTTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAACCAAGTGACCAATATAGCTCGAGCTATATTGGTCACTTGGTTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAACCAAGTGACCAATATAGCTCGAGCTATATTGGTCACTTGGTTGT-3'

Other clones with same target sequence:

(none)