Construct: shRNA TRCN0000238086

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001127324.1-820s21c1
DNA Barcode:
GAACTTCCTATCACGGGTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ercc1 (13870)
Gene Description:
excision repair cross-complementing rodent repair deficiency, complementation group 1
Transcript:
RefSeq NM_001127324.1 (CURRENT)
Match location:
Position 820 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238086)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACTTCCTATCACGGGTATC
Hairpin Sequence:
5'-CCGG-GAACTTCCTATCACGGGTATC-CTCGAG-GATACCCGTGATAGGAAGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACTTCCTATCACGGGTATCCTCGAGGATACCCGTGATAGGAAGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACTTCCTATCACGGGTATCCTCGAGGATACCCGTGATAGGAAGTTC-3'

Other clones with same target sequence:

(none)