Construct: shRNA TRCN0000238105

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145602.2-714s21c1
DNA Barcode:
GCCGCAGAGACCTTGATATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ndrg4 (234593)
Gene Description:
N-myc downstream regulated gene 4
Transcript:
RefSeq NM_145602.2 (NON-CURRENT)
Match location:
Position 714 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238105)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGCAGAGACCTTGATATTA
Hairpin Sequence:
5'-CCGG-GCCGCAGAGACCTTGATATTA-CTCGAG-TAATATCAAGGTCTCTGCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGCAGAGACCTTGATATTACTCGAGTAATATCAAGGTCTCTGCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGCAGAGACCTTGATATTACTCGAGTAATATCAAGGTCTCTGCGGC-3'

Other clones with same target sequence:

(none)