Construct: shRNA TRCN0000238142

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011424.2-7377s21c1
DNA Barcode:
GAAAGGCACTCATGGGTAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ncor2 (20602)
Gene Description:
nuclear receptor co-repressor 2
Transcript:
RefSeq NM_011424.2 (NON-CURRENT)
Match location:
Position 7377 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238142)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGGCACTCATGGGTAAAT
Hairpin Sequence:
5'-CCGG-GAAAGGCACTCATGGGTAAAT-CTCGAG-ATTTACCCATGAGTGCCTTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGGCACTCATGGGTAAATCTCGAGATTTACCCATGAGTGCCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGGCACTCATGGGTAAATCTCGAGATTTACCCATGAGTGCCTTTC-3'

Other clones with same target sequence:

  1. TRCN0000296045