Construct: shRNA TRCN0000238211

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010797.2-1048s21c1
DNA Barcode:
TGATCAGGCTCCGCAAGTTAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mid1 (17318)
Gene Description:
midline 1
Transcript:
RefSeq NM_010797.2 (NON-CURRENT)
Match location:
Position 1048 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238211)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATCAGGCTCCGCAAGTTAG
Hairpin Sequence:
5'-CCGG-TGATCAGGCTCCGCAAGTTAG-CTCGAG-CTAACTTGCGGAGCCTGATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATCAGGCTCCGCAAGTTAGCTCGAGCTAACTTGCGGAGCCTGATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATCAGGCTCCGCAAGTTAGCTCGAGCTAACTTGCGGAGCCTGATCA-3'

Other clones with same target sequence:

(none)