Construct: shRNA TRCN0000238255

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008668.2-993s21c1
DNA Barcode:
GAGAGGATCTTCCGGAGTTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nab2 (17937)
Gene Description:
Ngfi-A binding protein 2
Transcript:
RefSeq NM_008668.2 (CURRENT)
Match location:
Position 993 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238255)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAGGATCTTCCGGAGTTTC
Hairpin Sequence:
5'-CCGG-GAGAGGATCTTCCGGAGTTTC-CTCGAG-GAAACTCCGGAAGATCCTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAGGATCTTCCGGAGTTTCCTCGAGGAAACTCCGGAAGATCCTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAGGATCTTCCGGAGTTTCCTCGAGGAAACTCCGGAAGATCCTCTC-3'

Other clones with same target sequence:

(none)