Construct: shRNA TRCN0000238256

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008668.2-1040s21c1
DNA Barcode:
ATCCCTGTTGAAGCTGAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nab2 (17937)
Gene Description:
Ngfi-A binding protein 2
Transcript:
RefSeq NM_008668.2 (CURRENT)
Match location:
Position 1040 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238256)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCCCTGTTGAAGCTGAATAA
Hairpin Sequence:
5'-CCGG-ATCCCTGTTGAAGCTGAATAA-CTCGAG-TTATTCAGCTTCAACAGGGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCCCTGTTGAAGCTGAATAACTCGAGTTATTCAGCTTCAACAGGGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCCCTGTTGAAGCTGAATAACTCGAGTTATTCAGCTTCAACAGGGAT-3'

Other clones with same target sequence:

(none)