Construct: shRNA TRCN0000238289

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177773.3-2172s21c1
DNA Barcode:
ATGCCTTGCCTCCTAACTAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
4933408B17Rik (271508)
Gene Description:
RIKEN cDNA 4933408B17 gene
Transcript:
RefSeq NM_177773.3 (NON-CURRENT)
Match location:
Position 2172 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238289)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGCCTTGCCTCCTAACTAAG
Hairpin Sequence:
5'-CCGG-ATGCCTTGCCTCCTAACTAAG-CTCGAG-CTTAGTTAGGAGGCAAGGCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGCCTTGCCTCCTAACTAAGCTCGAGCTTAGTTAGGAGGCAAGGCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGCCTTGCCTCCTAACTAAGCTCGAGCTTAGTTAGGAGGCAAGGCAT-3'

Other clones with same target sequence:

(none)