Construct: shRNA TRCN0000238304

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080819.1-7749s21c1
DNA Barcode:
CTTTATAGTATGGCGAGTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arid1a (93760)
Gene Description:
AT rich interactive domain 1A (SWI-like)
Transcript:
RefSeq NM_001080819.1 (CURRENT)
Match location:
Position 7749 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238304)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTATAGTATGGCGAGTTAA
Hairpin Sequence:
5'-CCGG-CTTTATAGTATGGCGAGTTAA-CTCGAG-TTAACTCGCCATACTATAAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTATAGTATGGCGAGTTAACTCGAGTTAACTCGCCATACTATAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTATAGTATGGCGAGTTAACTCGAGTTAACTCGCCATACTATAAAG-3'

Other clones with same target sequence:

(none)