Construct: shRNA TRCN0000238386

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006598.2-614s21c1
DNA Barcode:
GCGGGTCCTACTACATGATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC12A7 (10723)
Gene Description:
solute carrier family 12 member 7
Transcript:
RefSeq NM_006598.2 (NON-CURRENT)
Match location:
Position 614 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238386)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGGTCCTACTACATGATAT
Hairpin Sequence:
5'-CCGG-GCGGGTCCTACTACATGATAT-CTCGAG-ATATCATGTAGTAGGACCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGGTCCTACTACATGATATCTCGAGATATCATGTAGTAGGACCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGGTCCTACTACATGATATCTCGAGATATCATGTAGTAGGACCCGC-3'

Other clones with same target sequence:

(none)