Construct: shRNA TRCN0000238428

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011878.2-5467s21c1
DNA Barcode:
GTGTGCTTACAATGCTCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tiam2 (24001)
Gene Description:
T cell lymphoma invasion and metastasis 2
Transcript:
RefSeq NM_011878.2 (CURRENT)
Match location:
Position 5467 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238428)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTGCTTACAATGCTCATTT
Hairpin Sequence:
5'-CCGG-GTGTGCTTACAATGCTCATTT-CTCGAG-AAATGAGCATTGTAAGCACAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTGCTTACAATGCTCATTTCTCGAGAAATGAGCATTGTAAGCACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTGCTTACAATGCTCATTTCTCGAGAAATGAGCATTGTAAGCACAC-3'

Other clones with same target sequence:

(none)